A Novel Method Offers Better & Faster Human Antibodies Production

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An electron microscopy image shows an antibody-secreting plasma cell generated using antigen- and CpG-coated nanoparticles.
An electron microscopy image shows an antibody-secreting plasma cell generated using antigen- and CpG-coated nanoparticles. Credit: Sanjuan Nandin et al., 2017


An international team of scientists has developed a method to rapidly produce specific human antibodies in the laboratory. The technique.... could speed the production of antibodies to treat a wide range of diseases and facilitate the development of new vaccines.

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Antibodies are produced by the body's B cells to fight off infections by bacteria, viruses, and other invasive pathogens. When an individual B cell recognizes a specific pathogen-derived "antigen" molecule, it can proliferate and develop into plasma cells that secrete large amounts of antibody capable of binding to the antigen and fending off the infection.

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Researchers have sought to replicate this process in the laboratory to produce specific antibodies from B cells isolated from patient blood samples.

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A team of researchers... have been able to produce specific human antibodies in the laboratory by treating patient-derived B cells with tiny nanoparticles coated with both CpG oligonucleotides and the appropriate antigen. With this technique, CpG oligonucleotides are only internalized into B cells that recognize the specific antigen, and these cells are therefore the only ones in which TLR9 is activated to induce their proliferation and development into antibody-secreting plasma cells.

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The team successfully demonstrated their approach using various bacterial and viral antigens, including the tetanus toxoid and proteins from several strains of influenza A. In each case, the researchers were able to produce specific, high-affinity antibodies in just a few days. Some of the anti-influenza antibodies generated by the technique recognized multiple strains of the virus and were able to neutralize its ability to infect cells.

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News Reference: A Novel Method Offers Better Antibodies Production

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