Product Review: ISOLATE II RNA Mini Kit



 ISOLATE II RNA Mini Kit is specially designed for fast and efficient isolation of extremely pure total RNA from a wide variety of samples. The kit is compatible with mammalian tissues, cultured cells, bacterial cells, yeast, bacteria, biological fluids, paraffin embedded tissue, RNAlater® treated samples, as well as other starting materials.

Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae.

The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA. RNase-free DNase I is supplied with the kit.

There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted.

RNA isolated with the ISOLATE II RNA Mini Kit shows excellent performance in downstream applications, such as reverse transcription, one-step real-time PCR. Northern blot analysis, microarrays and RNA protection assays.

Features and Benefits 

  • Rapid protocol - 30 min/6 preps 
  • High purity RNA - typical A260/A280 ratio 1.9 - 2.1 
  • High quality - RNA integrity number (RIN) >9 
  • Filters (shredders) included - to enhance sample homogenization 
  • DNase I included - for complete removal of genomic DNA

  • Mammalian tissues 
  • Eukaryotic cells Bacterial cells 
  • Yeast 
  • Biological fluids (cell-free) 
Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR.

Customer Testimonials

"I find that the 260/280 and 260/230 ratios with this kit (ISOLATE II RNA Mini Kit) are excellent and reproducible. I previously used the Qiagen RNeasy kit, but the Bioline kit is both cheaper and gives better quality RNA."
 - Newcastle University, UK

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