Product Review: ISOLATE II Genomic DNA Kit

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ISOLATE II Genomic DNA Kit

This kit is designed for the rapid and efficient isolation of highly pure genomic DNA using the latest developments in silica membrane spin column technology.

ISOLATE II Genomic DNA Kit uses the latest developments in silica-membrane spin column technology. The isolation process combines fast lysis of the starting material with Proteinase K, followed by specific binding of DNA directly to the membrane in a spin column. Subsequent washing steps result in elution of high-quality DNA.

The isolated DNA is suitable for downstream molecular biology applications such as multiplex PCR, Southern blotting, real-time PCR, sequencing, restriction analysis and microarrays.  

Features and Benefits 
  •  Rapid protocol - ~20 minutes (after lysis) 
  • High-purity genomic DNA - typical A260/A280 ratio 1.7 – 1.9 
  • High yield - up to 35µg genomic DNA 
  • Broad spectrum kit - with over 17 protocols

Applications

Its application is basically to the isolation of genomic DNA from: 
  •  Animal tissue and rodent tails 
  • Paraffin embedded tissue 
  • Clinical and forensic samples 
  • Bacterial cells 
  • Eukaryotic cells (yeast, cultured mammalian)

Customer Testimonials

I have used this kit to extract genomic DNA from mice tails (approx. 0.6 cm) for genotyping. Usually a sufficient amount of quality DNA (0.5 to 3 micrograms @ 5-30 ng/µl) from the mice tails was extracted through using this kit- to carry out genotyping. An input of 50 ng for each DNA sample was used for genotyping. This kit comes with very clear, user friendly instructions provided and provides efficient, reliable and cost effective isolation of DNA- overall a great kit for genomic DNA extraction and downstream analysis.
- Wilson Wong, University of Sydney, Camperdown , Australia

The ISOLATE II Genomic DNA Kit is extremely sensitive. It produced high-purity results and I can start direct PCR without contamination. The Agarose gel was clear. I like this product.
 - Andre Gauchel 



 

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